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  • Knowledge of laboratory raising of spruce web-spinning sawfly (Cephalcia abietis (L.)) larvae in diapause and their parasitoids
  • 作者: Kanecka, P
  • literature id: 29476
  • catalog nub: TPL_KANECK1994KOLRO25002550
  • 文献库: Taxapad收录文献
  • type: article
  • publication name: Lesnictvi (Prague)
  • publish date: 1994-01-01
  • pages: 250-255
  • volume: 40
  • issue: 6
  • 创建时间: 2021-03-02 15:00:32
  • create by: zxmlmq (admin)
  • comment:

    none. Laboratory raising of diapause stages of spruce web-spinning sawfly is very problematic. Raised larvae often die of injuries and various diseases, even in the cases in which they would be able to survive in normal living conditions (intact development in the ground gal(cry). This high mortality rate is particularly due to negative impacts of a change in the environment when in the raised individuals their natural physiological processes have been disturbed. The paper is a summary of accumulated many-year knowledge of laboratory raisin of this important spruce pest, while it can also be a guide for those who would be interested to know how to raise relatively successfully the larvae of spruce web-spinning sawfly in diapause, or parasitoids of the family Ichneumonidae obtained from them. The following conditions of successful raising have been determined for both groups on the basis of long-range trials. The larvae of spruce web-spinning sawfly should be raised separating them into eonymphs and young pronymphs and older pronymphs, while the first group (eonymphs and young pronymphs) requires temperatures of 0 to +2 degree C for three weeks, followed by two weeks of the temperatures 18-20 degree C until the pupal eye is formed (Fig. 1). The older pronymphs (with the fully developed pupal eye) are put in the conditions of mild frost (maximally to -3 degree C, usually -2 degree C) for three weeks, followed by two weeks of the temperatures 0 to +2 degree C until they assume the pupal stage. The pupae are better to be put to conical tubes containing filter paper and covered with monofile and wrapped up in a PE bag with a larger piece of moist cotton-wool to keep the required moisture content. The pupae are then kept until the imago stage at a laboratory temperature (18-20 degree C). The larvae of ichneumon flies must be put to an ellipsoid gallery of the spruce web-spinning sawfly which its larvae make in litter, just before they leave the body of the host larva, while the specific position of the dead larva in the gallery should be provided for (Fig. 2). When the cocoon is formed, it shall be transferred to test tubes (similarly Re the pupae of the spruce web-spinning sawfly) which are then placed to cold conditions (= to +2 degree C) for three weeks, followed by two weeks at the temperature up to 18 - 20 degree C and three weeks in the cold conditions again (0 to +2 degree C). Consequently, the raised cocoons must be subjected to the same temperature cycle as the older pronymphs (mild frost for three weeks, cold for two weeks), for the time of three months at least. After that time, the test tubes with cocoons will be put to the conditions of laboratory temperature in which they will be left until the pupa is transformed into the imago stage. Synchronous development with the host was observed in the larvae of ichneumon flies leaving the larvae (Tab. I) of C. abietis. In two cases the development was asynchronous (as an exception), one case was the ichneumon fly Homaspis rufinus (Grav.). The moment when the larva of the ichneumon fly leaves the body of its host apparently depends on the species. The ichneumon larvae consume the pigment of the pupal eye and of the larval eye (tapetum nigrinum spot) in the pronymphs and eonymphs, while the remaining integuments of the larvae (left by the parasitoid) contain only epicuticle, exocuticle and traces of endocuticle.

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