Frugivorous flies have been acquiring great economic importance in coffee crop because they cause premature dropping of coffee beans and significantly decrease the quality of the beverage. The coffee plant is also a natural reservoir of Tephritidae species that infest fruits of economic importance. This study evaluated the influence of the shaded and unshaded coffee systems, under organic management, on the natural infestation of fruits of Coffea arabica L. var. Icatu Amarelo by frugivorous flies. An experiment in completely randomized design was carried out in Valenca, RJ, Brazil, with two treatments (shaded coffee with Musa sp. and Erithrina verna Veil. and unshaded coffee monoculture) and four replicates. A I kg-sample of maturing fruits per plot was harvested in Mach 2004 to evaluate infestation and identify the flies. The mean infestation index was significantly higher in the shaded coffee system. Four species of Tephritidae (Ceratitis capitata (Wiedemann), Anastrepha fraterculus (Wiedemann), Anastrepha obliqua (Macquart) and Anastrepha sororcula Zucchi), and six of Lonchaeidae (Neosilba pendula (Bezzi), Neosilba pseudopendula (Korytkowski and Ojeda), Neosilba certa (Walker), Neosilba glaberrima (Wiedemann), Neosilba n.sp.9 and Neosilba n.sp.10) were recovered from coffee fruits. Nine parasitoid species were obtained, six belonging to Braconidae (Asobara anastrephae (Muesebeck), Doryctobracon areolatus (Szepligeti), Doryctobracon brasiliensis (Szepligeti), Utetes anastrephae (Viereck), Opius bellus Gahan and Opius sp.), and three to Figitidae (Aganaspis pelleranoi (Brethes), Dicerataspis flavipes (Kieffer) and Odontosema anastrephae Borgmeier). The total percent parasitism was 8.8% and 12.4% in the shaded and unshaded coffee systems, respectively. Tephritoidea (Cyclorrhapha) : Host, Parasite Braconidae (Ichneumonoidea) : Parasite Figitidae (Cynipidae) : Parasite Animals and man; Disturbance by man; Commercial activities; Parasites diseases and disorders; Parasites; Insect parasites; Hosts; Insect hosts; Habitat; Man made habitat; Land zones; Neotropical region; South America Braconidae; Figitidae; Farming and agriculture; Organic shaded & unshaded coffee systems; Dipteran hosts; Parasitism levels; shaded & unshaded coffee systems comparison; Tephritoidea; Cultivated land habitat; Brazil; Rio de Janeiro, Valenca Tephritoidea; Farming and agriculture; Organic shaded & unshaded coffee systems; Hymenopteran parasites; Plant hosts; Infestation indices; shaded & unshaded systems comparison; Coffea arabica; Cultivated land habitat; Brazil; Rio de Janeiro, Valenca none