Response to temperature of Aphidius colemani Viereck (Hymenoptera, Braconidae, Aphidiinae) from three climatic regions of Minas Gerais state, Brazil. The parasitoid Aphidius colemani Viereck, 1912 presents higher mortality at constant temperatures above 25[degree]C, and probably it is the cause of the failure on the biological control of Aphis gossypii Glover, 1877 when this parasitoid is used at higher temperatures in greenhouses. The aim of this work was to evaluate the influence of different temperatures on individuals of A. colemani from different climatic regions of Minas Gerais state, Brazil. Individuals of A. colemani were collected in the localities of Juramento, Lavras and Sao Gotardo, and reared in laboratory (22[plus or minus]2[degree]C, 70[plus or minus]10% RH and 12h photophase) for three generations in the host A. gossypii, on cucumber plants. One mated female of A. colemani, 24-48 h old, was released for one hour, in a Petri dish (15cm) with 20 nymphs of 2nd instar of A. gossypii on a leaf disk of cucumber (4 cm of diameter) laid on a layer of agar-water (1%). The parasitized aphids were kept at 16, 19, 22, 25 and 28[plus or minus]1[degree]C, 70[plus or minus]10% RH and 12h photophase. The emergence rates of individuals from Juramento (65.9 and 35.4%) and Sao Gotardo (71.4 and 47.6%) were lower compared to those from Lavras (87.1 and 80.9%) at 16 and 28[degree]C. The more suitable temperature for the development of A. colemani from Lavras was higher than those for individuals from Juramento and Sao Gotardo. The individuals from Lavras showed highest emergence at 28[degree]C, and this demonstrated the existence of individuals of A. colemani with tolerance to higher temperatures. These results open news perspectives on the use of different strains of A. colemani on biological control of A. gossypii in protected cultivation. Aphidius colemani (Aphidiidae) : Parasite Aphis gossypii (Aphidoidea) : Host Animals and man; Control; Biological control; Life cycle and development; Parasites diseases and disorders; Parasites; Insect parasites; Hosts; Insect hosts; Abiotic factors; Physical factors; Land zones; Neotropical region; South America Aphidius colemani; Control of; Aphis gossypii (Hemiptera); Development; Hemipteran hosts; Biological control; implications of development & temperature relationships; Aphis gossypii; Climate and weather; Temperature; Brazil; Minas Gerais Aphis gossypii; Control by; Aphidius colemani (Hymenoptera); Hymenopteran parasites; Biological control; implications of parasite development & temperature relationships; Aphidius colemani; Climate and weather; Temperature; Brazil; Minas Gerais none