none LEPIDOPTERA-; HYMENOPTERAN-PARASITES; Bracon hebetor; Insecticidal activity of parasite venom gland toxins Bracon hebetor; TOXINS-AND-VENOMS; Venom gland toxins; VENOM-GLANDS; Toxins, purification & characterization; Insecticidal activity on lepidopteran hosts; LEPIDOPTERAN-HOSTS; Insecticidal activity of venom gland toxins The potency of venom from Bracon hebetor against lepidopterous larvae has been known for over 40 years, but previous attempts to purify and characterize individual protein toxins have been largely unsuccessful. Three protein toxins were purified from venom of this small parasitic wasp and the amino acid sequences of 22-31 consecutive residues at the amino-terminus were determined. These relatively large toxins (apparent molecular mass 73 kDa) were labile under many isolation techniques, but anion-exchange chromatography allowed purification with retention of biological activity. Two purified toxins were quite insecticidal (LD, lt 0.3 mu-g/g) when injected into six species of lepidopterous larvae. On a molar basis, one toxin (Brh-I) has the highest known biocidal activity against Heliothis virescens (LD-50 = 2 pmol/g).