- Preliminary results on in vitro rearing of the endoparasitoid Cardiochiles nigriceps from egg to second instar
- 作者: Pennacchio, F.; Vinson, S.B. and Tremblay, E
- literature id: 37465
- catalog nub: TPL_PENNAC1992PROIV20902160
- 文献库: Taxapad收录文献
- type: article
- publication name: Entomologia Experimentalis et Applicata
- publish date: 1992-01-01
- pages: 209-216
- volume: 64
- issue: 3
- 创建时间: 2021-03-02 15:00:32
- create by: zxmlmq (admin)
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comment:
none Cardiochiles nigriceps; REARING-TECHNIQUES; In vitro method for embryo & early larval stages; EMBRYO-DEVELOPMENT; LARVAL-DEVELOPMENT; In vivo & in vitro development times. The composition of an artificial medium and technical procedures used for in vitro rearing of the endophagous larval parasitoid Cardiochiles nigriceps Viereck (Hymenoptera, Braconidae), from post-germ band egg to the 2nd instar larva, are described. Amino acids, carbohydrates, salts, and vitamins were supplied in defined amounts as an aqueous solution which, when supplemented with 20 mg/ml of bovine albumin, 5 mg/ml of lactalbumin (enzymatic hydrolysate), 20% (v/v) fetal bovine serum, 20% (v/v) milk and 10% (v/v) chicken egg yolk, allowed for parasitoid growth and molting to the 2nd instar. Molting to the final instar was never observed.
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