Different species of parasitoids associated with flies collected on human faeces in ltumbiara County, Goias State, Brazil, were identified. Traps of black tin can with baits and sand were used. Fly pupae were obtained, sifted for extraction, and placed individually into gelatin capsules until emergence of adult flies and/or parasitoids. The preference of species for their host wasevaluated by the Chi-square test, at 5.0% probability. The total percentage of parasitism was 21.9%. From March 2001 to April2002, 180 specimens of parasitoids were collected in 823 pupae of dipterans. Variable numbers of specimens of 6 different species of parasitoids were obtained from pupae of infested flies: 22 specimens of Gnathopleura quadridentata Wharton (Braconidae),21 specimens of Brachymeriapodagrica (Fabricius) (Chalcididae), 23 specimens of Hemencyrtus herbertii Asmead (Encyrtidae), 1 specimen of Paraganaspis egeria Diaz and Gallardo (Eucoilidae), 13 specimens of Nasonia vitripennis (Walker) and 100 specimensof Pachycrepoideus uindemiae (Rondani) (Pteromalidae). The total percentages of parasitism for Gnathopleura quadridentata, Brachymeria podagrica, Hemencyrtus herbertii, Poraganaspis egeria, Nasonia vitripennis and Pachycrepoideus uindemiae were2.7%, 2.5%, 2.8%, 0.1%, 1.5% and 12.1%, respectively. This is the first report on the occurrence of Brachymeria podagrico(Fabricius) parasitizing pupae of Ophyra sp. in Brazil. Nutrition; Parasites diseases and disorders; Parasites; Insect parasites; Hosts; Insect hosts; Land and freshwater zones; Neotropical region; South America Diptera; Diet; Human faeces; Hymenopteran parasites recorded; Hymenopteran parasites; Records from hosts feeding on human faeces; Brazil; Goias; Itumbiara County, records from species feeding on human faeces Hymenoptera; Dipteran hosts; Records from hosts feeding on human faeces; Brazil; Goias; Itumbiara County; Records from dipteran hosts feeding on human faeces none