none Larval-pupal apolysis of Anastrepha suspensa is inhibited when superparasitized (up to 7 parasites/host) by the solitary endoparasite Biosteres longicaudatus . Ecdysone 20-monooxygenase activity was similar in superparasitized hosts and controls and could not explain the significantly (P < 0.05) high ecdysteroid titres, especially in 36-48-h-old hosts. Superparasitized A. suspensa were unable to initiate larval-pupal apolysis due presumably, to: (a) their inability to secrete moulting gel and (b) high ecdysteroid titres that may also have inhibited the synthesis of a new cuticle. Biochemistry; Chemical composition; Integument; Endocrinology; Life cycle and development; Development; Parasites diseases and disorders; Parasites; Insect parasites; Hosts; Insect hosts; Host parasite relationships Anastrepha suspensa (Tephritidae); Steroid content; Cuticle; Synthesis ability relations with superparasitism by hymenopteran; Hormones; Ecdysteroids, function & effect of superparasitism by hymenopteran; Ecdysis; Inhibition due to superparasitism by hymenopteran; Metamorphosis; Larval, effect of superparasitism by hymenopteran; Hymenopteran parasites; Biosteres longicaudatus; Superparasitism effect on host integument & ecdysteroid content; Prevalence; Biosteres (Hymenoptera), relations with host cuticle synthesis & ecdysis; Reaction of host to parasite; Integumentary changes & ecdysteroid content; Relations with superparasitism by Biosteres longicaudatus (Hymenoptera) Biosteres longicaudatus (Braconidae); Dipteran hosts; Anastrepha suspensa; Superparasitism effect on host integument & ecdysteroid content; Prevalence; Anastrepha suspensa (Diptera), effect on host integument & ecdysteroids; Reaction of host to parasite; Dipteran host integument & ecdysteroid content changes