Phenoloxidose (PO) is a major component of the insect immune system. The enzyme is involved in encapsulation and melanization processes as well as wound healing and cuticle sclerotization. PO is present as an inactive proenzyme, prophenoloxidase (PPO), which is activated via a protease cascade. In this study, we have cloned a full-length PPO1 EDNA and a partial PPO2 EDNA from the Indianmeal moth, Pladia interpunctella (Hubner) (Lepidoptera: Pyralidae) and documented changes in PO activity in larvae paralyzed and parasitized by the ectoparasitoid Habrabracon hebetor (Say) (Hymenoptera: Braconidae). The EDNA for PPO1 is 2,748 bp and encodes a protein of 681 amino acids with a calculated molecular weight of 78,328 and pl of 6.41 containing a conserved proteolytic cleavage site found in other PPOs. P interpunctella PPO1 ranges from 71-78% identical to other known lepidopteran PPO-1 sequences. Percent identity decreases as comparisons are made to PPO-1 of more divergent species in the orders Diptera (Au-48; As-49; and Sb-60%) and Coleoptera (Tm-58; Hd-50%). Paralyzation of host larvae of P interpunctella by the idiobiont H. hebetor results in an increase in phenoloxidase activity in host hemolymph, a process that may protect the host from microbial infection during self-provisioning by this wasp. Subsequent parasitization by H. hebetor larvae causes a decrease in hemolymph PO activity, which suggests that the larval parasitoid may be secreting an immunosuppressant into the host larva during feeding. Published 2005 Wiley-Liss, Inc. Plodia interpunctella (Pyralidae) : Host Habrobracon hebetor (Braconidae) : Parasite Biochemistry; Proteins; Genetics; Parasites diseases and disorders; Parasites; Insect parasites; Hosts; Insect hosts; Immunology and repair mechanisms Habrobracon hebetor; Lepidopteran hosts; Molecular cloning of proenzyme cDNA & enzyme activity in paralyzed & parasitized larvae; Plodia interpunctella Plodia interpunctella; Enzymes; Proenzyme cDNA & enzyme activity; Molecular genetics; Proenzyme cDNA; Hymenopteran parasites; Molecular cloning of proenzyme cDNA & enzyme activity in paralyzed & parasitized larvae; Habrobracon hebetor; Immune like reactions none