This study reports the first occurrence of Coptera haywardi Loiacono as a pupal parasitoid of fruit-infesting Tephritidae in Brazil. We reared this diapriid from fruits of Eugenia unifiora (Surinam cherry) infested by Anastrepha fraterculus (Wiedemann) and/or Anastrepha sororcula Zucchi. We also identified two other species of pupal parasitoids: Pachrycrepoideus vindemmiae Rondani and Spalangia endius Walker (Pteromalidae). Four species of larval-pupal parasitoids were also recorded: Doryctobracon areolatus (Szepligeti), Utetes (Bracanastrepha) anastrephae (Viereck), Opius bellus Gahan (Braconidae) and Aganaspis pelleranoi (Brethes) (Figitidae). Parasites diseases and disorders; Parasites; Insect parasites; Hosts; Insect hosts; Land and freshwater zones; Neotropical region; South America Tephritidae; Hymenopteran parasites; Parasite records; Brazil; Rio de Janeiro; Seropedica; Hymenopteran parasite records Hymenoptera; Dipteran hosts; Tephritidae; Parasite records; Brazil; Rio de Janeiro; Seropedica; Parasites of Diptera, records Coptera haywardi (Diapriidae); Dipteran hosts; Anastrepha; New pupal parasitoid record; Brazil none